De 'Phelix Phage Borrelia test' en Borrelia miyamotoi en Relapsing Fever.
EngelandLyme Disease UK (LDUK); Bron
https://lymediseaseuk.com/2021/05/17/dr ... teulieres/Teulières - 'Online Seminar
27th May 2021: Phage Testing for Lyme Disease' - slides; Bron
https://lymediseaseuk.com/wp-content/up ... y-2021.pdf ..Phelix Charity and Leicester University
developed a Borrelia SL, RF and a specific B. miyamotoi Phage PCR tests already validated in
early and late stage patients and healthy volunteers,
showing >90% sensitivity and
100% specificity due to confirmatory sequencing..
..Results of the Phage BorreliaTests Done on Late stage patients
Methods
Borrelia burgd sl and a specific B. miyamotoi and Relapsing fever Phages PCR Tests were performed on over 2200 samples (all late stage) from various European countries and from USA. All results were verified by sequencing.
Results:
34% of the tested samples were negative, 66% positive among which 64% Borrelia miyamotoi; 25% RF
60%-70% van de patiënten uit
Nederland en andere verschillende landen in
Europa en uit
Amerika hebben
B. miyamotoi/Relapsing Fever?
Onafhankelijke validatie en verificatie van de experimentele en niet-gevalideerde 'Phelix Phage Borrelia test' én klinische onderzoeken (clinical trials), dubbelblind gerandomiseerd placebo gecontroleerd onderzoek (Randomized Controlled Trial (RCT)) hebben (nog) niet plaatsgevonden; Bron
viewtopic.php?f=5&t=2322&start=840#p28262EngelandPublic Health England - Lyme Disease; Bron
https://www.gov.uk/government/collectio ... d-analysisTeken onderzoek EngelandBMC
Research Parisites & Vectors
Publicatie
26 augustus 2021Open Access -
'Occurrence of tick-borne pathogens in questing Ixodes ricinus ticks from Wester Ross, Northwest Scotland' by Fanny Olsthoorn, Hein Sprong, Manoj Fonville, Mara Rocchi, Jolyon Medlock, Lucy Gilbert & Jaboury Ghazoul; Bron
https://parasitesandvectors.biomedcentr ... 21-04946-5 en Bron
https://pubmed.ncbi.nlm.nih.gov/34446082/..Abstract
Background: Lyme borreliosis and other tick-borne diseases emerge from increased interactions between humans, other animals, and infected ticks. The risk of acquiring a tick-borne infection varies across space and time, so knowledge of the occurrence and prevalence of pathogens in ticks can facilitate disease diagnosis in a specific area and the implementation of mitigation measures and awareness campaigns. Here we identify the occurrence and prevalence of several pathogens in Ixodes ricinus ticks in Wester Ross, Northwest Scotland, a region of high tourism and tick exposure, yet data-poor in terms of tick-borne pathogens..
..Methods: Questing I. ricinus nymphs (n = 2828) were collected from 26 sites in 2018 and 2019 and tested for the presence of tick-borne pathogens using PCR-based methods. Prevalence was compared with other regions of Scotland, England, Wales, and the Netherlands..
..Results: Anaplasma phagocytophilum (4.7% prevalence), Borrelia burgdorferi sensu lato (s.l.) (2.2%), Babesia from clade X (0.2%), Rickettsia helvetica (0.04%), and Spiroplasma ixodetis (0.4%) were detected, but no Neoehrlichia mikurensis, Borrelia miyamotoi, or Babesia microti. Typing of A. phagocytophilum using a fragment of the GroEL gene identified the presence of both ecotype I and ecotype II. Genospecies identification of Borrelia burgdorferi s.l. revealed B. afzelii (53% of infected nymphs), B. garinii (9%), B. burgdorferi sensu stricto (7%), and B. valaisiana (31%). We found similar prevalence of A. phagocytophilum in Wester Ross as in the Netherlands, but higher than in other parts of Great Britain. We found lower B. burgdorferi s.l. prevalence than in England or the Netherlands, and similar to some other Scottish studies. We found higher prevalence of B. valaisiana and lower prevalence of B. garinii than in other Scottish studies. We found S. ixodetis at much lower prevalence than in the Netherlands, and R. helvetica at much lower prevalence than in England and the Netherlands..
..Conclusions: As far as we know, this is the first description of S. ixodetis in Great Britain. The results are relevant for disease surveillance and management for public and veterinary health. The findings can also aid in designing targeted public health campaigns and in raising awareness among outdoor recreationists and professionals..
..Discussion
Neoehrlichia mikurensis, Borrelia miyamotoi, and Babesia microti
Although we did not observe N. mikurensis, B. miyamotoi, or Ba. microti in questing nymphs from Wester Ross, this cannot necessarily be interpreted as a complete absence of enzootic circulation of these pathogens, because the prevalence of some of these pathogens can be low, for example, 0.1% for N. mikurensis in regions of Denmark [87], 0.6% for Ba. microti in Poland [88] and 1.5% in the UK (ticks pulled from dogs) [89], and 0.8% for B. miyamotoi in regions of Germany [90] and 0.5% in England (Table 1). In our sample of 2828 ticks, a prevalence of 0.1% would mean that there would be an expectation of around three ticks that test positive in our sample size. Therefore, it could be that the pathogens are present but were undetected, especially if the circulation of the pathogens is very localised or occurs in habitats insufficiently monitored in this study. In a separate study, also in Wester Ross, we did find Ba. microti in one engorged tick pulled from a wood mouse (Apodemus sylvaticus) (Olsthoorn et al. unpubl.). Neoehrlichia mikurensis has so far not been found in any study that tested for it in Great Britain [36, 37], so there is a realistic possibility that this pathogen is not circulating in Scotland.
We tested 8282 questing nymph and adult ticks, and it is possible that a tiny proportion of the ticks analysed may have been non-ricinus, although this is highly unlikely, as previous studies from Scotland identified 100% of questing ticks (9883 in total) to be I. ricinus [20,21,22]..
Table 1 - Wester Ross (Northwest Scotland), Other regions of Scotland, England and Wales, The Netherlands ; Bron
https://parasitesandvectors.biomedcentr ... 5/tables/1Preprints with The Lancet
14 januari 2022
Study -
'The Prevalence of Borrelia miyamotoi in Ixodes Ticks and Humans in the Northern Hemisphere: A Systematic Review and Meta-Analysis' by Dieuwertje Hoornstra, Tal Azagi, Jacqueline A. van Eck, Alex Wagemakers, Joris Koetsveld, René Spijker, Alexander E. Platonov, Hein Sprong, Joppe W. Hovius; Bron
https://papers.ssrn.com/sol3/papers.cfm ... id=4008931..Abstract
Background: Various studies have evaluated infection of Ixodes ticks and humans with the relapsing fever spirochete Borrelia miyamotoi. However, it has never been assessed systematically. This systematic review and meta-analysis examines B. miyamotoi prevalence in Ixodes ticks and humans, and the disease it can cause in the Northern hemisphere..
..Methods: PubMed and Web of Science were searched up to March 1th, 2021. Publications assessing Ixodes tick infection using data from 2011 onwards were eligible, whereas no time limitation was placed on human reports. B. miyamotoi test positivity ratios were extracted. A random effects-model was used to calculate estimated proportions (ES) with 95% confidence interval (CI)..
..Findings: Included articles reported on 165 637 questing ticks, 45 608 unique individuals, and 504 well-described human cases. In ticks, the highest prevalence was observed in Ixodes persulcatus (2·8%, 95%CI 2·4-3·1%) and the lowest in I. pacificus ticks (0·7, 95%CI 0·6-0·8%). The overall seroprevalence in humans was 4·4% (95%CI 2·8-6·3%), with a significantly (p<0·001) higher seroprevalence in the high risk group (4·6%, 95%CI 2·6-7·1%), participants with confirmed or suspected Lyme borreliosis (4·8%, 95%CI 1·8-8·8%), and individuals suspected of another tick-borne disease (11·9%, 95%CI 5·6-19·9%), as compared to healthy controls (1·3%, 95%CI 0·4-2·8%). Individuals suspected of another tick-borne disease were significantly more often B. miyamotoi-PCR positive than the high risk group (p=0·0246), with individuals in Asia more likely to be positive than those in the United States of America (OR 14·63, 95%CI 2·80-76·41)..
..Interpretation: Our findings demonstrate Borrelia miyamotoi disease should be considered an emerging infectious disease, especially in North America and Asia. Prospective studies and increased awareness are required to obtain further insights into the burden of disease..
SchotlandThe Scottish ParliamentPublic Petitions Committee Meeting
De
Schotse Petitie is afgerond en gesloten op
17 november 2021; Bron
https://petitions.parliament.scot/petitions/PE1662 Nieuwe site van het Schotse Parlement; Bron
https://www.parliament.scot/Tweede rondetafel gesprek op
22 juni 2021Tick-borne Illness Campaign Scotland; Bron
https://www.facebook.com/ticscotland/Our second Scottish Government round-table meeting took place this week. This time we talked about Lyme testing and treatment and touched on research. Read our report here:
http://www.ticscotland.org.uk/news/roundtable-20210622The minutes from the first meeting are being finalised and we have permission to distribute the final version.
Thanks once again to those doctors and other healthcare professionals who give up their time to speak up for patients.
Schotse VWS30 oktober 2020 - Brief van Schotse VWS - PE1662/PP Minister for Health, Sport and Wellbeing submission of 30 October 2020; Bron
https://www.parliament.scot/S5_PublicPe ... 662_PP.pdf Scottish Lyme Disease and Tick-borne Infections Reference Laboratory (SLDTRL).
..SLDTRL is committed to investigating new testing methods and technologies and is constantly aiming to adapt and improve the service provided. However, as the NICE guidelines state, tests should only be used if they have been sufficiently validated, and this validation should include peer-reviewed published evidence on the test methodology, its relation to Lyme disease and independent reports of performance. To their knowledge, there are no other tests available that fulfil these criteria..
6 oktober 2020 - PE1662/OO Petitioner submission of 6 October 2020I; Bron
http://www.parliament.scot/S5_PublicPet ... 662_OO.pdf..The Phelix Phage Borrelia test is a new test, developed in conjunction with Leicester University,which is designed to test for bacterial presence and is not dependent 2on host antibody production5. We would like to see this test assessed for routine use in Lyme disease testing in Scotland...
6 december 2020 - PE1662/SS Petitioner submission of 6 December 2020; Bron
https://www.parliament.scot/S5_PublicPe ... 662_SS.pdf..As stated in previous submissions, only 5 of the 300 known species of Borrelia are currently tested for in Scotland.
Borrelia miyamotoi is known to exist in Scotland
and scientists believe it is responsible for a significant proportion of illness *5.
SLDTRL’s test for Borrelia miyamotoi is not ISO accredited or routinely used, leaving many patients undiagnosed. This may also be the case for other recently discovered species. There is also strong evidence that many Lyme disease patients are also infected with tick-borne co-infections, but SLDTRL has no ISO accredited tests any co-infections..
