..'The phb-PCR test has been evaluated using analytical, clinical and tick samples, and its accuracy and reliability have been validated and peer-reviewed, with results published in scientific literature'..?
Is het gestelde juist? De Phelix Phage Borrelia test is een experimentele en niet-gevalideerde test. Ter verificatieBron viewtopic.php?f=5&t=2595&start=520#p30645 ..De References (Referenties)29, 15 en 19 worden genoemd als medisch én wetenschappelijk bewijs?
29. Shan, J., M.R. Clokie, and L. Teulières. Phage-based detection of borreliosis and means therefor, US11739388B2, PCT/GB2017/053323, WO 2018/083491 2018.
15. Shan, J., et al., Targeting Multicopy Prophage Genes for the Increased Detection of Borrelia burgdorferi Sensu Lato (s.l.), the Causative Agents of Lyme Disease, in Blood. Front. Microbiol., 2021. 12: 651217.
19. Shan, J., et al., Combining citizen science and molecular diagnostic methods to investigate the prevalence of Borrelia burgdorferi s.l. and Borrelia miyamotoi in tick pools across Great Britain. Front Microbiol, 2023. 14: p. 1126498..
Reference 19: En opmerkelijke verschillen tussen de publicaties van 12 juni 2023 en van 30 november 2023 'Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing'Pre-prints.org; Bron https://www.preprints.org/
Publicatie 12 juni 2023..This version is not peer-reviewed..
Review - 'Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing' by Ying Jia1, Tatjana Mijatovic2, Louis Teulières3, Martha Clokie1, Jinyu Shan1*; Bron https://www.preprints.org/manuscript/202306.0816/v1
..Abstract: Tick-borne diseases are a growing concern worldwide, affecting both human and animal populations. Ticks are known to harbor a wide range of pathogens and are considered one of the most important vectors of disease. Lyme disease, caused by Borrelia burgdorferi sensu lato, is the most common tick-borne disease in the US and Europe. However, accurate diagnosis of Lyme disease can be challenging due to the complex immune evasion strategies employed by Borrelia species and the limitations of existing diagnostic tests. To address this issue, researchers are exploring novel approaches, including the use of bacteriophages as diagnostic tools. Bacteriophages are highly specific and offer advantages over traditional methods for detecting bacteria, including Borrelia.
In particular, the use of multicopy bacteriophages as molecular markers for Borrelia detection is a promising approach that may provide greater sensitivity than targeting single-copy bacterial genes. Nonetheless, the task of identifying trace amounts of bacteriophages in blood samples necessitates attention, and scientists are devising innovative techniques to surmount this hurdle. In summary, employing bacteriophages as a diagnostic tool for tick-borne diseases, such as Lyme disease, by specifically targeting free circulating bacteriophages in blood samples, offers significant potential for enhancing patient outcomes and public health..
..Conflicts of Interest: The authors declare no conflict of interest..
..Author Contributions:..**LT provided valuable advice on LD clinical diagnosis and treatment..
European Society of Medicine; Bron https://esmed.org/
Publicatie 30 november 2023
Open Access - 'Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing' by Ying Jia1, Tatjana Mijatovic2, Louis Teulières3, Martha Clokie1, Jinyu Shan1*; Bron https://esmed.org/MRA/mra/article/view/4650/99193547476 en Bron https://esmed.org/MRA/mra/article/view/4650
..Abstract
Tick-borne diseases are a growing concern worldwide, affecting both human and animal populations. Ticks are known to harbour a wide range of pathogens and are considered one of the most important vectors of diseases. Lyme disease, caused by Borrelia burgdorferi sensu lato, is the most common tick-borne disease in the US and Europe. However, accurate diagnosis of Lyme disease can be challenging due to the complex immune evasion strategies employed by Borrelia species and the limitations of existing diagnostic tests. To address this issue, researchers are exploring novel approaches, including the use of bacteriophages as diagnostic tools. Bacteriophages are highly specific and offer advantages over traditional methods for detecting bacteria, including Borrelia.
In particular, the use of multicopy bacteriophages as molecular markers for Borrelia detection is a promising approach that may provide greater sensitivity than targeting single-copy bacterial genes. Nonetheless, the task of identifying trace amounts of bacteriophages in blood samples necessitates attention, and scientists are devising innovative techniques to surmount this hurdle. In summary, employing bacteriophages as a diagnostic tool for Lyme disease, by specifically targeting free circulating bacteriophages in blood samples, offers significant potential for enhancing patient outcomes and public health. However, additional rigorous scientific validation is required to definitively ascertain the efficacy and accuracy of using a phage-based methodology for detecting Borrelia in blood samples.
..Abstract
..However, additional rigorous scientific
..Introduction
..However, all these advantages
..Conflicts of Interest: The authors declare specific affiliations that may constitute a potential conflict of interest. The second author is affiliated with R.E.D. Laboratories, a commercial entity that offers testing using the “PHELIX PHAGE TEST.” Phelix Research and Development provided substantial support for these studies, and one of the authors is a member of Phelix R&D. Furthermore, the corresponding author is affiliated with Phelix Research and Development. While these affiliations exist, they were maintained in a consultative and advisory capacity with the intention of furthering understanding and development in LD diagnostics. No legal conflict of interest is claimed. However, the connections between the authors and the entities involved in the development and offering of the Phelix Phage Test are openly acknowledged. This disclosure is made to ensure transparency and allow readers to fully consider the context and any potential interests related to this work..
..Author Contributions:..**LT provided valuable advice on LD clinical diagnosis and treatment..
Er is géén sprake van onafhankelijk onderzoek en de Case-study kan niet dienen als medisch én wetenschappelijk bewijs. De auteurs en Louvet zijn leden (trustees, mede-oprichters én investeerders!) van Phelix Research & Development (Engeland); Bron http://phelix.info/en/about/team/ & Phelix France (Frankrijk); Bron https://zb14927861.phelix.org.uk/team/ en Bron https://zb14927861.phelix.org.uk/about-us/ en hebben een financieel belang!