Zijn er ervaringen met de Phelix Phage Borrelia test?

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Di 15 Okt 2024, 17:28

Vervolg op; Bron viewtopic.php?f=38&t=2435&start=220#p30746

10 oktober 2024: Red Laboratories - New article: Diagnosis and treatment of Borrelia miyamotoi Phage Borrelia; Bron https://www.redlabs.com/2024/10/diagnos ... motoi.html
..We are thrilled to announce the new article on Phage test: “Successful diagnosis and treatment of Borrelia miyamotoi in a patient with joint and muscle pains, ME/CFS and cognitive dysfunction following tick bites: a case report.”

This article is a case report detailing the successful diagnosis and treatment of Borrelia miyamotoi disease (BMD), a type of tick-borne relapsing fever (TBRF), in a patient suffering from joint and muscle pain, cognitive dysfunction, and chronic fatigue after multiple tick bites. Diagnosing BMD is particularly challenging due to its similarity to Lyme disease (LD) and the lack of a characteristic rash that can help diagnose LD. In this case, standard diagnostic tests (like ELISA and antibody tests) failed to detect any infections, including LD, Bartonella, or Anaplasma.

However, a phage-based PCR (phb-PCR) test, which detects bacterial phages rather than just bacterial DNA, was able to identify Borrelia miyamotoi. This allowed the patient to receive appropriate treatment, including antibiotics and intravenous vitamin therapy, resulting in a significant improvement in her symptoms.

:arrow: Importance of the Article for Understanding Lyme and Tick-borne Diseases:
- Diagnostic Challenges: The report highlights how current Lyme disease tests can be unreliable when other tick-borne infections like BMD are involved. It emphasizes that negative test results for Lyme and other infections don't rule out the presence of pathogens like Borrelia miyamotoi.
- Phage-based PCR: The study demonstrates the potential of phb-PCR as a more sensitive and accurate diagnostic tool for detecting Borrelia miyamotoi, especially when standard bacterial tests fail. This is crucial for correctly diagnosing and treating complex cases of tick-borne illnesses.
- Symptom Overlap: It illustrates the significant overlap in symptoms between LD and TBRF, making it difficult for clinicians to distinguish between these diseases without advanced diagnostic methods. This complicates treatment and can lead to chronic, unresolved symptoms if misdiagnosed.
- Treatment Success: The case provides a hopeful example of how proper diagnosis can lead to effective treatment, improving patient outcomes in otherwise difficult-to-treat cases.

For more information, please visit our Redlabs website Phage page.

Please find bellow three more publications for the Phage Borrelia Test:
:arrow: 1. https://www.ncbi.nlm.nih.gov/pmc/articl ... 651217.pdf
:arrow: 2. https://www.ncbi.nlm.nih.gov/pmc/articl ... 126498.pdf
:arrow: 3. https://esmed.org/MRA/mra/article/view/4650/99193547476 ..
Is het :arrow: door Red Laboratories gestelde juist? Ter verificatie lees en leer meer; Bron viewtopic.php?f=38&t=2435&start=220#p30746 en Bron viewtopic.php?f=38&t=2751#p30600

Successful Diagnosis and Treatment of Borrelia miyamotoi in a Patient with Joint and Muscle Pains, ME/CFS, and Cognitive Dysfunction following Tick Bites???: Publicatie; Bron viewtopic.php?f=5&t=2595&start=520#p30644 En :arrow: Reference 29; Bron viewtopic.php?f=5&t=2595&start=520#p30645 En :arrow: Reference 15 Bron viewtopic.php?f=5&t=2595&start=520#p30648 En :arrow: teken-onderzoek; Bron viewtopic.php?f=5&t=2595&start=520#p30650 En :arrow: Reference 19; Bron viewtopic.php?f=5&t=2595&start=520#p30651

..'However, a phage-based PCR (phb-PCR) test, which detects bacterial phages rather than just bacterial DNA, was able to identify Borrelia miyamotoi'..?
..'This allowed the patient to receive appropriate treatment, including antibiotics and intravenous vitamin therapy, resulting in a significant improvement in her symptoms'..?

Is het gestelde juist? :arrow: Met de kostbare experimentele en niet-gevalideerde Phelix Phage Borrelia test kan géén diagnose Borrelia burgdorferi sensu lato (s.l) en of B. miyamotoi/Relapsing Fever worden gesteld! zie geschiedenis; Bron viewtopic.php?f=5&t=2595&start=340#p30016
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Di 05 Nov 2024, 14:07

Vervolg op; Bron viewtopic.php?f=38&t=2435&start=220#p30746

Stand van zaken - European Patent Office (EPO) - WO2022072615 (EP21876463/EP4221827) 'Method of manipulating phages within the body to attack infectious target cells'; Bron https://register.epo.org/application?number=EP21876463 en; Bron https://register.epo.org/application?nu ... ab=doclist

Stand van zaken 29 en 30 oktober 2024: - European Patent Office (EPO); Bron https://www.epo.org/
..29.10.2024: Application deemed to be withdrawn (Non-reply to Written Opinion)..
..30.10.2024: New entry: Application deemed to be withdrawn: despatch of communication + time limit..

Examination procedure: 29.10.2024: Despatch of communication that the application is deemed to be withdrawn, reason: reply to the Extended European Search Report/Written Opinion of the International Searching Authority/International Preliminary Examination Report/Supplementary international search report not received in time..
Er is een (niet verlengbaar) uitstel verleend van 2 maanden voor het beantwoorden van de door de Onderzoekscommissie van de European Patent Office (EPO) gestelde vragen. Er moet voor 29 december 2024; Bron https://register.epo.org/application?do ... &npl=false worden gereageerd.
Interessant gaat worden met welke 'bewijzen' (voor onder andere de 'EM signature' en de 'BES findings'; Bron viewtopic.php?f=38&t=2435&start=220#p30682 ..) Jernigan (natuurarts, géén onderzoeker & wetenschapper) van Biologix Center (Verenigde Staten) zal gaan komen.
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Wo 01 Jan 2025, 16:55

Vervolg

Stand van zaken - European Patent Office (EPO) - WO2022072615 (EP21876463/EP4221827) 'Method of manipulating phages within the body to attack infectious target cells'; Bron https://register.epo.org/application?number=EP21876463 en; Bron https://register.epo.org/application?nu ... ab=doclist

Stand van zaken 24 december 2024: - European Patent Office (EPO); Bron https://www.epo.org/
..24.12.2024: New entry: Receipt of request for further processing..
Interessant gaat worden met welke 'bewijzen' (voor onder andere de 'EM signature' en de 'BES findings'; Bron viewtopic.php?f=38&t=2435&start=220#p30682 ..) Jernigan (natuurarts, géén onderzoeker & wetenschapper) van Biologix Center (Verenigde Staten) zal gaan komen.
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Do 02 Jan 2025, 16:35

Stand van zaken - European Patent Office (EPO) - WO2022072615 (EP21876463/EP4221827) 'Method of manipulating phages within the body to attack infectious target cells'; Bron https://register.epo.org/application?number=EP21876463 en; Bron https://register.epo.org/application?nu ... ab=doclist

Stand van zaken 24 december 2024: - European Patent Office (EPO); Bron https://www.epo.org/
..24.12.2024: Amended description with annotations..
..24.12.2024: Amended claims with annotations..
De gemachtigde (advocaat octrooirecht; Barker Brettell LLP) heeft namens Jernigan op 24 december 2024 een reactie gegeven. De claims (5 pagina's); Bron https://register.epo.org/application?do ... &npl=false en de description (3 pagina's); Bron https://register.epo.org/application?do ... &npl=false zijn gewijzigd. De vorige ingediende claims en description van 16 november 2023 waren; Bron viewtopic.php?f=38&t=2435&start=200#p29915
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Vr 03 Jan 2025, 16:20

Vervolg op; Bron viewtopic.php?f=38&t=2435&start=200#p29929

Red Laboratories (België) is een commercieel laboratorium en heeft géén ISO 15189 accreditatie van het BELAC België (de Belgische Accreditatie-instelling - Medische laboratoria (MED); Bron https://economie.fgov.be/nl/themas/kwal ... atoria-med
Lees en leer meer
; Bron viewtopic.php?f=38&t=2751#p30583 en; Bron viewtopic.php?f=38&t=2751#p30594 en; Bron viewtopic.php?f=38&t=2435&start=220#p30236 en; Bron viewtopic.php?f=38&t=2435&start=220#p30237

Het zou erg interessant zijn als een groep onafhankelijke geaccrediteerde medische laboratoria (ISO 15189); Bron https://www.rva.nl/medische-laboratoria/ met Ringonderzoek; Bron https://www.rva.nl/nieuws/accreditatie- ... al-erkend/ de experimentele en niet-gevalideerde Phelix Phage Borrelia test (PCR) van Red Laboratories (België) zouden kunnen gaan verifiëren en valideren.

Ter verificatie: 9 juni 2024: Het lukt de wetenschapper/onderzoeker Shan; Bron https://phelix.info/about/team/ niet om subsidie gelden te krijgen voor zijn 'Startup'; Bron viewtopic.php?f=38&t=2435&p=30466#p30465 en; Bron viewtopic.php?f=38&t=2435&start=220#p30236 en; Bron viewtopic.php?f=38&t=2435&start=220#p30237


Stand van zaken Phelix Research & Development (Engeland); Bron https://phelix.info/about/team/ & Phelix France (Frankrijk); Bron https://zb14927861.phelix.org.uk/team/ periode t/m 31 december 2023; Bron https://zb14927861.phelix.org.uk/about-us/
..During the year, the charity received income totalling £45.0045 and incurred expenditure of £41.390, giving a net surplus position of £3.655.

Phelix R&D recognised income of £45.001 from RedLab Belgium form the commercialisation of the Phage based test for the year 2023..

..Activity update
Activity 2023:
- Red Labs sold 1,292 rests in 2023 versus 1,750 in 2022, a drop of 26% YoY.
- Phelix R&D negotiated with the University of Leicester to reduce its contribution to the research. The University found some internal resources to fund itself for the first half of 2023, while the Charity financed the research for the second half of 2023.
- The research primarily focused on understanding why the traditional Borrelia strain (BB31) is less prevalent in Europe than in de US.
- The University of Leicester kept developing new primers for Bartonella strains.
- The University of Leicester, in collaboration with Red Laboratories, focused on implementing a series of triplicate qPCR assays for Borrelia burgdorferi sensu lato.

Phelix R&D in the partnership of the University of Leicester to publish a research paper titled 'Combining Combining Citizen Science and Molecular Diagnostic Methods to Investigate the Prevalence of Borrelia burgdorferi s.l. and Borrelia miyamotoi in Tick Pools Across Great Britain'; Bron https://www.frontiersin.org/journals/mi ... 26498/full

The University of Leicester, Phelix R&D and Red Laboratories have finalized and published a research article in the European Society of Medicine: Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing; Bron https://esmed.org/MRA/mra/article/view/4650

Phelix R&D and Red Laboratories attended the ILADS conference in Boston in October 2023. They presented Phelix R&D's research to medical professionals and a panel of university researchers.

..Whilist the trustees are aware of the need to build reserves for a substaniable future. This is not considered necessary at this time due to the support of the trustees..

Stand van zaken: European Patent Office (EPO) - Federated register; Bron https://register.epo.org/application?nu ... =federated
In Europa is in veel landen het ** :arrow: patent(octrooi) EP3535412 (WO2018083491A1) - 'Phage-based detecition of Borreliosis and means therfor'; (inventors Clokie, Shan en Teulières 'Phelix Phage Borrelia test'); Bron https://register.epo.org/application?nu ... n&tab=main ) komen te vervallen (in 2021, 2022, 2023 en 2024).
'Lapse of the patent in a contracting state' - Event history; Bron https://register.epo.org/application?nu ... &tab=event
Bij enkel de landen België; Bron https://bpp.economie.fgov.be/fo-eregist ... ?locale=en Frankrijk; Bron https://data.inpi.fr/brevets/EP3535412 Engeland; Bron https://www.ipo.gov.uk/p-ipsum/Case/App ... 17795037.5 is het patent(octrooi) niet vervallen.

Kunnen de artikelen 'Combining Combining Citizen Science and Molecular Diagnostic Methods to Investigate the Prevalence of Borrelia burgdorferi s.l. and Borrelia miyamotoi in Tick Pools Across Great Britain' en 'Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing' dienen als medisch én wetenschappelijk bewijs?
Lees en leer meer
en ter verificatie; Bron viewtopic.php?f=5&t=2595&start=520#p30651 en Bron viewtopic.php?f=5&t=2595&start=530#p30652 En; Bron viewtopic.php?f=38&t=2435&start=200#p29593 en; Bron viewtopic.php?f=5&t=2595&start=330#p30000

Vervolg op: 'Stand van zaken Phelix Research & Development (Engeland) & Phelix France (Frankrijk) periode t/m 31 december 2021'; Bron viewtopic.php?f=38&t=2435&start=180#p29157
Stand van zaken Phelix Research & Development (Engeland); Bron http://phelix.info/en/about/team/ & Phelix France (Frankrijk); Bron https://zb14927861.phelix.org.uk/team/ periode t/m 31 december 2022; Bron https://zb14927861.phelix.org.uk/about-us/

..During the year, the charity received income totalling £60.205 and incurred expenditure of £80.501, giving a net loss position of £20.269.

Phelix R&D recognised income of £52.908 from RedLab Belgium form the commercialisation of the Phage based test for the year 2022..

..Activity update
Activity 2022:
- Improving the sensitivity of the Phage Based borrelia test with our partner in Belgium, RedLabs, who are in charge of the commercialisation of the Test.
- Exploring new technologies in order to automate the DNA extraction from biological samples.
- University of Leicester and Phelix Research and Development: Trying to redesign a Phage Based test for Bartonella sp. as the previous test did not show promising sensitivity.
- University of Leicester and Phelix Research and Development: working on the next research paper on Borrelia Miyamotoi.
- Finalising the documents for the filing of the Patent of the Borrelia phage based test: UK, USA, EU (Patent attorney Murgitroyd law firm)..

..Whilist the trustees are aware of the need to build reserves for a substaniable future. This is not considered necessary at this time due to the support of the trustees..

Interessant is hoe de verdere ontwikkelingen zullen gaan. Er is een tekort van £20.269 (€23.403) ontstaan. De trustees, mede-oprichters en investeerders (Teulières en Louvet (Charity trustees are the people who ultimately exercise control over and are legally responsible for the charity. If the charity is a company these people may also be known as directors or board members)) moeten verschillende leningen (totaal 2022: £382.246 in 2021: £378.492) zien terug te verdienen in de komende jaren.
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~

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Roxy
Berichten: 7884
Lid geworden op: Wo 29 Okt 2014, 12:14

Re: Zijn er ervaringen met de Phelix Phage Borrelia test?

Berichtdoor Roxy » Di 14 Jan 2025, 15:00

Stand van zaken - European Patent Office (EPO) - WO2022072615 (EP21876463/EP4221827) 'Method of manipulating phages within the body to attack infectious target cells'; Bron https://register.epo.org/application?number=EP21876463 en; Bron https://register.epo.org/application?nu ... ab=doclist

Stand van zaken 13 januari 2025: - European Patent Office (EPO); Bron https://www.epo.org/
..13.01.2025: Decision to allow further processing..
..02.01.2025: Amendment by applicant..
..02.01.2025: New entry: Fee payment for request for further processing..
De Onderzoekscommissie van het European Patent Office (EPO) heeft besloten het onderzoek voort te zetten.
~ I may not be there yet, but I'm closer than I was yesterday ~
~ There is nothing more beautiful than a rainbow but it takes both rain and sunshine to make a rainbow ~
~ So encourage each other and build each other up - Positive connections ~


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