Biofilm

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Henriëtte
Berichten: 1671
Lid geworden op: Di 19 Mei 2015, 10:05

Re: Biofilm

Berichtdoor Henriëtte » Vr 13 Sep 2019, 13:01

The Emerging Role of Microbial Biofilm in Lyme Neuroborreliosis; 03-12-2018

LNB is, in many cases, responsive to appropriate antimicrobial therapy and the clinical improvement sustained by the antibiotic treatment provide further evidence for the direct contribution of B. burgdorferi in disease pathogenesis. However, the chronic persistence, the frequent reoccurrence of LNB and the ability of B. burgdorferi to tolerate multiple cycles of antibiotic treatment is strongly suggestive for the formation of biofilm or biofilm-like protective structure (20–23). Indeed, different studies have shown that B. burgdorferi can switch from a motile to a stationary status, in which the cells are embedded within a biofilm matrix (22). B. burgdorferi biofilms have been observed both in vitro and in human infected skin tissues (22, 23). These structures express different mucopolysaccharides, particularly alginate, extracellular DNA and calcium, which are all typical markers of biofilm (22). The presence of biofilm may explain the low rate of Borrelia detection in the blood of infected patients as well as the ability of the spirochetes to evade the host immune system and resist the antibiotic therapy (21, 24–27).

This review investigates the differences in the epidemiology and clinical manifestations of LNB with particular emphasis on the pathogenetic role of B. burgdorferi biofilm in tissue adhesion, colonization and survival.


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287027/

Henriëtte

Sproetje
Berichten: 3160
Lid geworden op: Di 28 Okt 2014, 20:33

Re: Biofilm

Berichtdoor Sproetje » Vr 13 Sep 2019, 19:17

Biofilm Structures of Borrelia burgdorferi Not Only Display More Tolerance to Lyme Antibiotics But Also Cause More Severe Pathology In a Mouse Arthritis Model: Implications for Understanding Persistence, PTLDS and Treatment Failure

Jie Feng, Tingting Li, Ying Zhang
2018

https://www.biorxiv.org/content/10.1101/440461v2


…..
Here we isolated biofilm-like microcolony and planktopic form (spirochetal forms and round body) from stationary phase culture and found that the stationary phase planktonic form (SP) and microcolony form (MC) were much more tolerant to the current antibiotics for Lyme disease, doxycycline, ceftriaxone and cefuroxime than log phase spirochete form (LOG). In addition, we also compared the ability of the variant forms to cause disease in a mouse arthritis model. Surprisingly, the MC in particular and the SP caused a more severe arthritis with an earlier onset of inflammation and joint swelling than LOG. MC-infected mice showed significant joint swelling as early as 9 days post-infection, while the LOG and SP did not cause significant swelling.
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VerlorengezondheidM
Berichten: 2006
Lid geworden op: Za 19 Sep 2015, 23:59
Locatie: Tilburg
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Re: Biofilm

Berichtdoor VerlorengezondheidM » Ma 11 Nov 2019, 00:38

Polymicrobial Biofilms In Chronic Infectious Disease

Polymicrobial biofilms are associated with various chronic infections including diabetic foot ulcers, chronic obstructive pulmonary disease, anaerobic soft tissue infections, cystic fibrosis lung infection, and chronic tonsillitis, to name a few.

These diverse microbial communities are in essence an ecosystem. Both host and microorganisms recognize and respond to each other, as do the various members of the microbial community. In this way, microorganisms co-evolve, evade immune clearance and so persist for many months or years. Permanent or transient genetic changes allow bacteria to adapt to the host environment, in some cases dampening acute immune responses that would otherwise clear infection, and perpetuating low-grade inflammation that promotes persistent colonization.
Synergistic behavior between different bacterial species can result in increased virulence and better colonization. Chronic polymicrobial infections are notoriously difficult to treat with conventional antimicrobial therapies. In part, this is due to the protective nature of the biofilm lifestyle, but may also be consequent to synergies between members of the microbial community that increase collective tolerance. This means that for many people with chronic infections, the clinical outcome is management rather than cure, with the aim of impairing progression. However, ultimately, the longer a chronic infection persists, the poorer the prognosis.


Ineffective or inaccurate diagnosis can hamper the successful treatment of chronic infections. Many routine isolation and identification strategies do not account for the polymicrobial, biofilm nature of chronic infections, nor are they optimal for giving an accurate representation of all the microorganisms present.

Longstanding questions about the role of polymicrobial community interactions in infection aetiology, virulence, persistence and antibiotic resistance, which were previously impossible to address, are starting to become answerable with new infection models and analytical approaches. It is therefore timely to bring together new literature that seeks to open the “black box” of polymicrobial biofilm using a variety of approaches.


Bron: Frontiersin, lees meer
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